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Applied Molecular Biology
作者
：
出版日期
：
2009
閱讀格式
：
PDF
ISBN
：
9789881828415
Even though molecular biology has long been a basic tool in biomedical research, scientists still face the question of why certain molecular biology methods are used for certain experiments. To unlock the mystery, one must first understand the principles behind the methods. Unfortunately, very few molecular biology books have successfully provided satisfactory explanations. This book intends to fill this void by offering topics ranging from basic knowledge to the current state of the art in applied molecular biology. The principles and applications related to each technique included in the text are all described in full detail.
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Chapter 1: Nucleic Acid Structure and Properties
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1.1: Nucleic Acid
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1.2: Ribose and Base
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1.3: Nucleoside
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1.4: Nucleotide
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1.5: Structure of DNA and RNA
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1.6: Physical Properties of DNA and RNA
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Chapter 2: Replication， Transcription and Translation
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2.1: Introduction
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2.2: DNA Replication
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2.3: Transcription 20
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2.4: Capping of Pre- mRNA
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2.5: Splicing of Pre- mRNA
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2.6: Polyadenylation of Pre- mRNA
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2.7: Translation
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2.8: Regulation of Translation
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2.9: Ribosome
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2.10: Translation Factors
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2.11: Eukaryotic Translation
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2.12: Internal Ribosome Entry Site
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Chapter 3: Isolation of DNA and RNA
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3.1: Plasmid DNA Isolation
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3.2: Genomic DNA Isolation
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3.3: Total RNA Isolation
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3.4: Isolation of Poly-A+ mRNA
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3.5: Determination of the Quality and Quantity of DNA or RNA
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Chapter 4: Enzymes Used in Molecular Biology
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4.1: Types of Enzymes used in Molecular Biology
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4.2: Degradation Enzymes
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4.3: Discovery of Restriction Enzymes
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4.4: Classifi cation of Restriction-Modifi cation Enzymes
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4.5: Nomenclature of Restriction Enzymes
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4.6: DNA Ends Generated by Restriction Enzymes
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4.7: Factors Infl uencing the Activity of Restriction Enzymes
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4.8: Common Restriction Enzymes
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4.9. Isoschizomers
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4.10: Methyltransferases
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4.11: Other Degradation Enzymes
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4.12: RNA Polymerases
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4.13: DNA Polymerases
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4.14: Modifi cation Enzymes
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Chapter 5: Promoter and Operon
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5.1: Promoter
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5.2: Transcription Terminators of Prokaryotic Genes
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5.3: Eukaryotic Gene Transcription
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5.4: Elements Affecting Eukaryotic Gene Transcription
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5.5: Operon
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5.6: Lac Operon
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5.7: Suppression of Lac Operon
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5.8: Activation of Lac Operon
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Chapter 6: Electrophoresis
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6.1: Types of Electrophoresis
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6.2: Electrophoresis of RNA
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6.3: Resolution limits of Electrophoresis Gels
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6.4: Pulsed-fi eld Gel Electrophoresis
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6.5: Preparation of DNA Samples for PFGE
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6.6: Factors Affecting the Effi ciency of PFGE
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Chapter 7: Cloning
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7.1: Requi red Elements of Cloning Vectors
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7.2: pBR322 and pUC19 Vectors
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7.3: Isolation of DNA Fragments for Cloning
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7.4: Determination of Quantity and Quality of DNA Fragments
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7.5: Determination of Insert to Vector Ratio
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7.6: Spin Dialysis
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7.7: Transformation
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7.8: Screening of Transformants
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7.9: Ligation of DNA Fragments with Vector
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7.10: Prevention of Vector Self Ligation
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7.11: Use of Tailing， Adaptors and Linkers
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7.12: E. coli Genotypes
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Chapter 8: Nucleic Acid Hybridization
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8.1: Defi nition and Application of Nucleic Acid Hybridization
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8.2: Southern Hybridization
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8.3: Probe Labeling
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8.4: Preparation of RNA Probes
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8.5: Non-radioactive Probes
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8.6: Pre hybridization
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8.7: Stringency of Hybridization
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8.8: Northern Hybridization
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8.9: Types of Probes
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Chapter 9: Library
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9.1: Definition and Types of Library
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9.2: Life Cycle of Lambda Bacteriophage
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9.3: Lambda Vectors
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9.4: Transcription Regulation of Lambda Bacteriophage
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9.5: Induction of Lambda Lysogen
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9.6: Factors affecting the Life Cycle of Lambda Phage
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9.7: Clear Mutants of Lambda Bacteriophage
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9.8: Property and Application of λgt10
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9.9: Property and Application of λgt11
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9.10: Generalized and Specialized Transducers
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9.11: cDNA Synthesis
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9.12. Packaging of Lambda Bacteriophage
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9.13: In Vitro Lambda Packaging
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9.14: Screening of cDNA Libraries
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9.15: Unidirectional cDNA Cloning
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9.16: Subtractive cDNA Library
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9.17. Differential cDNA Library Screening
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Chapter 10: Restriction Mapping
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10.1: Verifi cation of Recombinant Plasmids
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10.2: Identifi cation of Restriction Sites on a Plasmid
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Chapter 11: DNA Sequencing
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11.1: Principles of DNA Sequencing
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11.2: Maxam and Gilbert DNA Sequencing Method
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11.3: Sanger’s DNA Sequencing Method
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11.4: DNA labeling in Sanger’s Sequencing Method
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11.5: Properties of M13 Bacteriophage
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11.6. Development of M13 Vectors
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11.7: Development of Phagemids
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11.8: Common Sequencing Problems
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11.9: Cycle Sequencing
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11.10: Automated DNA Sequencing
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11.11: Pyrosequencing
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11.12: In vivo Excision of pBluescript from Lambda ZAP
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Chapter 12: Cloning Vectors
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12.1: Cloning Potential of Various Vectors
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12.2: EMBL3 and EMBL4 Vectors
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12.3: Cosmid
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12.4: Yeast Artifi cial Chromosome
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12.5: Bacterial Artifi cial Chromosome
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12.6: P1 Artifi cial Chromosome
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Chapter 13: Polymerase Chain Reaction
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13.1: Invention of Polymerase Chain Reaction
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13.2: PCR Primer Design
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13.3: Types of PCR
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13.4: PCR Contamination Problem
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13.5: PCR Controls
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13.6. Prevention of Non-specifi c PCR
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13.7: PCR Applications
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13.8: Cloning of PCR Products
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13.9: Other DNA Polymerases for PCR
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13.10: Other Methods for Nucleic Acid Amplifi cation
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Chapter 14: Expression of Recombinant Proteins
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14.1: Expression of Foreign Proteins in E. coli
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14.2: Inducible Promoters
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14.3: Fusion Proteins
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14.4: Isolation and Purifi cation of Recombinant Proteins
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14.5: Removal of Tags
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14.6: In-frame Fusion
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14.7: Construction of Expression Plasmids
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14.8: Detection of Expressed Proteins
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14.9: Phage Display
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14.10: Eukaryotic Expression Systems
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14.11: Regulation of Expression in Mammalian Cells
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14.12: Introduction of Recombinant Plasmid into Eukaryotic Cells
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14.13: Gateway Expression System
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Chapter 15: Mutagenesis， Transgenic Animals and RNA Interference
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15.1: Purpose of Mutagenesis
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15.2: Site-directed Mutagenesis
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15.3: Single-base Mutation
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15.4: Insertion and Deletion by PCR
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15.5: Advanced Methods for PCR Mutagenesis
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15.6: Homologous Recombination
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15.7: Insertion Mutagenesis by Homologous Recombination
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15.8: Single and Double Crossing Over
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15.9: Deletion Mutagenesis by Homologous Recombination
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15.10: Gene Replacement by Homologous Recombination
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15.11: Transgenic Animals
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15.12: RNA Interference
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Chapter 16: Viral Vectors
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16.1: Elements Requi red for Viral Vectors
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16.2: Retroviral Vectors
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16.3: Lentiviral vectors
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16.4: Adenovirus Vectors
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16.5: Adeno-associated Viral Vectors
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16.6: Herpes Simplex Virus Vector
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Chapter 17: Special Techniques
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17.1: Nuclear Run-on Assay
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17.2: Reporter Assay
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17.3: Electrophoretic Mobility Shift Assay
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17.4: DNase I Footprinting
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17.5: Chromatin Immunoprecipitation
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17.6: Two-hybrid System
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17.7: Three Hybrid System
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17.8: Primer Extension for Determination of Transcription Start Site
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17.9: S1 Nuclease Analysis
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17.10: RNase A Protection Assay
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17.11: Single Strand Conformation Polymorphism
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17.12: Heteroduplex Assay
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17.13: Denatu red Gradient Gel Electrophoresis
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17.14: Random Amplifi cation of Polymorphic DNA or Arbitrary Primed
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17.15: Amplifi ed Fragment Length Polymorphism
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17.16: mRNA Differential Display
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17.17: DNA Microarray
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17.18: Serial Analysis of Gene Expression
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17.19: Massively Parallel Signature Sequencing
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17.20: Sequencing by Oligo nucleotide Ligation and Detection
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17.21: Total Gene Expression Analysis
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17.22: Representational Difference Analysis
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- Glossar
- Index
- 出版地 ： 臺灣
- 語言 ： 英文
- DOI ： 10.6140/AP.9789881828415
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